badionotus, as well as on almost every other types of ocean cucumbers, browse throughout these taken advantage of types inside the Colombia has been limited to several studies on the variety and you will shipping [2, 16] and reproductive biology [17, 18]. Although not, there are still many holes when you look at the studies, particularly about their reproductive models and exactly how he is dependent on details including temperature, salinity, rain, and food access.
Today’s research identifies aspects of new reproductive biology of holothuroids We. badionotus and you may Isostichopus sp. about Santa Marta Area by using the gonadal index (GI) and histological examination of the gonads. This research is intended to enhance the current information about the brand new biology and you will ecology off water cucumbers native to the latest Colombian Caribbean Ocean and supply a technical reason for the introduction of government steps and maintenance with the investment.
dos. Material and techniques
Specimens away from Isostichopus sp. was basically obtained out of , when you find yourself specimens out of Isostichopus badionotus had been collected of throughout the Taganga Bay (11° 16′ ” N– 74° 11′ ” W) and you can Rodadero Bay (11° 12′ ” N– 74° 13′ ” W) on the Caribbean area for Santa Marta, Colombia (Fig step 1).
Additionally evaluates the influence off environment and you will chemical parameters (heat, rainfall, the fresh new percentage of natural carbon stuff on the sediment) on the reproductive biology and you will assesses the dwelling of the communities (frequency out of duration and you can pounds, intercourse proportion, indicate proportions and pounds to start with sexual maturity, fecundity)
Package into the inset A beneficial try magnified for the inset B; package during the inset B is magnified in the main panel. Source: Advice base INVEMAR.
As a result of the wide array of molds and colors that i kissbrides.com have a glimpse at the weblink. badionotus has, this study worried about this new distinct bacteria most abundant in well-known morphotype of this variety found in the research city, demonstrated by the Vergara ainsi que al. while i. badionotus morphotype II.
Month-to-month fourteen to help you 20 people of for each morphotype (I. badionotus and you can Isostichopus sp.) had been built-up from the snorkeling. For this reason, a couple of snorkelers swam a great transect 5 meters broad because of the around a thousand yards a lot of time, double. The original travels was made bordering the coast range additionally the 2nd well away of about 10 yards in the coastline range. Maximum search depth was ten m to own logistical and you will defense explanations and although it’s likely that you can find anyone within better length and depth, this could not be analyzed in the current studies. In October, zero collections was made as a result of the passage through of Hurricane Matthew, and therefore impacted the fresh new Colombian Caribbean during that few days. Most of the water cucumbers were actually packed in the plastic bags with seawater and you will transferred to the Aquaculture Laboratory of Universidad del Magdalena. New breadth out-of get each and every private and the sort of base they occupied had been submitted (categorized because the rocky, exotic, red coral, rock-red coral, rock-sand, rock-sand-coral). On top of that, skin deposit samples (with a density perhaps not more than 10 mm) had been drawn around the men and women to determine the brand new part of organic carbon blogs having fun with a mass spectrometer, Euro EA 3000 elemental analyzer (EuroVector). Salinity and you can h2o temperature was registered every month, if you are overall rainfall study was taken from the ideas of Simon Bolivar airport meteorological station of your own town of Santa Marta.
The folks have been sacrificed regarding laboratory from the setting them into the seawater at 4°C, that was and intended to come to a maximum state from contraction that allowed to reduce the error through the duration proportions, because of the skill that they have to increase otherwise package on have a tendency to. Then after the muscles measurements have been removed: full human body duration (TL), having fun with an adaptable 1-meters tape measure at the nearby 0.5 cm (once compromise of the thermal wonder the newest developed body got a c or S contour, and also the tape contoured the body, therefore, the full size ‘s the contour length); gonad frequency having fun with a graduated cylinder on nearby 0.1 cm 3 ; muscles wall lbs (BW), identified as the extra weight of the person rather than viscera otherwise gonad and you can gonad pounds (GW) at nearest 0.step one g. Gonad list (GI) are determined with the algorithm, GI = (GW/ BW)* one hundred [19, 20].